A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus

A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus.

A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chicken...

Detection of infectious bronchitis virus and specific anti- viral antibodies using a Concanavalin A-Sandwich-ELISA.

Concanavalin A-Sandwich ELISA (Con A-S-ELISA) was developed for the detection of infectious bronchitis virus (IBV) or chicken specific anti-viral antibodies. The antigen detection limit for the Con A-S-ELISA was 10(5,1) EID(50)/mL. Three homologous and four heterologous IBV strains were similarly detected. This assay was highly effective in detecting the virus after infected tissue homogenates ...

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

A serological survey for detection of avian infectious bronchitis virus antibodies in domestic village chickens in Esfahan, central Iran

Infectious bronchitis (IB) is a very contagious disease caused by a coronavirus (IBV). In chickens, thevirus affects the respiratory, reproductive, and urinary systems. This study was carried out to determine theseroprevalence of anti-IBV antibodies in domestic village chickens. Serum samples of 300 domestic villagechickens from Esfahan (centeral Iran) were collected and examined for the presen...

Development of blocking ELISA for detection of antibodies against avian influenza virus of the H7 subtype.

BACKGROUND AND PURPOSE The conventional method used for subtyping of antibodies against avian influenza viruses is hemagglutination inhibition (HI) test. However, the HI test is laborious and requires preparation of antigen from viable viruses that might be hazardous. The aim of this study was to develop a blocking enzyme-linked immunosorbent assay (B-ELISA) for detection of antibody of avian i...